In coordination with the Federal Government of Somalia, the strike happened at 10:30 a.m. local time about 125 miles northwest of Mogadishu, the country's capital.
The United States military has confirmed that over 100 Islamist militants were killed in an airstrike launched against the al-Shabaab group in Somalia.
A Somali intelligence official said US drone aircraft fired at least eight missiles at al-Shabab bases and training camps in Bur-Eylada, a village situated between the towns of Dinsor and Burhakaba in the Bay region.
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Al-Shabaab has pledged allegiance to al-Qaeda and is dedicated to providing safe haven for terrorist attacks throughout the world.
There have been 29 strikes acknowledged by the Pentagon so far this year.
On November 13, the Pentagon said U.S. forces had killed 40 Shabaab and Islamic State fighters in a series of Somalia strikes over several days.
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Al Shabaab spokesman Abdiasis Abu Musab denied the attack. The US pulled non-essential diplomatic staff out of Mogadishu this month after a threat against them.
The Takfiri militant group was forced out of the capital by the African Union troops in 2011 but still controls parts of the countryside and carries out attacks against government, military and civilian targets seemingly at will in Mogadishu and regional towns.
Earlier this month, the U.S. military also carried out its first strikes against Islamic State militants in Somalia and said it killed "several terrorists".
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The U.S. military this week also conducted two airstrikes in Libya against ISIS militants, according to a second Africom statement released Tuesday.