One of the trucks involved in the crash was hauling hazardous materials, which spilled out of the truck onto the roadway following the crash.
Indiana State Police said one semi hit two other tractor-trailers which were stopped due to an earlier crash.
Both lanes were immediately closed and remain so as clean up continues.
John Cena returns to the Kids' Choice Awards
Nickelodeon has announced that WWE Superstar John Cena has joined the voice cast as original character Baron Draxum. Cena will host the Nickelodeon Kids' Choice Awards for the second year in a row.
At last checked, all of the southbound lanes on I-65 are closed.
Eventually, the truck caught fire, posing what police called an "explosion risk" and prompting officials to issue a non-mandatory evacuation of the surrounding areas. ISP said they have yet to determine what if any charges will be brought against the driver that caused the crash Wednesday.
Westbound traffic was being diverted off I-80/94 at Grant Street, and eastbound traffic was being diverted off at Cline Avenue.
County is giving away radon test kits
The Environmental Protection Agency estimates that 21,000 lung cancer deaths every year are attributable to radon. Radon kits can be found at Home Depot, Wal-mart, Mr.D's, Bomgaar's and Mead Lumber in Sterling.
At around 10:30 a.m. the left lane reopened.
The Indiana Department of Environmental Management is cleaning up the chemicals, WLFI-TV reported. A private environmental cleanup company was also on hand to help offload the contents of the leaking trailer.
Both lanes are expected to be reopened by the afternoon.
Kane, De Gea snubbed in 2017 UEFA Team of the Year
De Bruyne's sparkling performance for Manchester City earned him a place in the team for the first time. Listen to the latest news on our 5 radio stations Legend FM, Viti FM, Radio Sargam, FM96 and Navtarang.